Karfi biyar:
● Kayan aiki da aka saita tare da cirewar acid nucleic da matakin tsarkakewa
● Kayan aiki da aka saita tare da tsarin hakar ultrasonic
● An saita kayan aiki tare da cikakken atomatik
● Kayan aiki da aka saita tare da ƙara girman zafin jiki
● Kayan aiki da aka saita tare da cikakken kayan aikin reagent
1.Shin ana buƙatar fitar da reagents na gano acid nucleic kuma a tsaftace su?
Ka'idar gano nucleic acid shine kamar haka: ƙarƙashin aikin farko, ana amfani da DNA polymerase don yin haɓaka amsawar sarkar akan samfuri DNA/RNA (yana buƙatar juyar da rubutun NA), sannan ana gano adadin siginar kyalli da aka saki don tantancewa. ko samfurin ya ƙunshi nucleic acid (DNA/RNA) na ƙwayoyin cuta da za a gano.
1) Samfuran da ba a fitar da su ba ko tsarkakewa na iya ƙunsar abubuwa da yawa waɗanda ke shafar sakamako na ƙarshe: nuclease (wanda zai iya narkar da maƙasudin nucleic acid kuma ya haifar da mummunan mummunan), protease (wanda zai iya rage DNA polymerase kuma ya haifar da mummunan rauni), ƙarfe mai nauyi. gishiri (wanda ke haifar da rashin kunnawa na synthase kuma yana haifar da tabbataccen ƙarya), ma acidic ko alkaline PH (wanda zai iya haifar da amsa ga gazawar), RNA mara cika (wanda ke haifar da gazawar juzu'i mara kyau).
2) Wasu samfurori suna da wuyar haɓakawa kai tsaye: Gram-positive da wasu parasites, saboda kaurin bangon cell ɗin su da sauran tsarin, idan ba su bi ta hanyar cirewar acid na nucleic da tsarin tsarkakewa ba, kayan da ba a cirewa ba na iya kasawa ga irin wannan. samfurori.
Don haka, ana ba da shawarar zaɓar kayan gwaji ko kayan aikin da aka haɗa tare da matakin cire sinadarin nucleic acid.
2. Chemical hakar ko jiki ultrasonic fragmentation hakar?
Gabaɗaya magana, ana iya amfani da hakar sinadarai zuwa mafi yawan pretreatment da tsarkakewa.Duk da haka, a cikin kwayoyin cutar Gram-positive masu kauri da wasu ƙwayoyin cuta, shi ma yanayin hakar sinadarai ba zai iya samun ingantattun samfuran nucleic acid ba, wanda ke haifar da gano mummuna na ƙarya.Bugu da ƙari, hakar sinadarai sau da yawa yana amfani da ma'aikata masu ƙarfi, idan elution ba cikakke ba ne, yana da sauƙi don gabatar da alkali mai ƙarfi a cikin tsarin amsawa, yana haifar da sakamako mara kyau.
Rarraba Ultrasonic yana amfani da murkushe jiki, wanda GeneXpert ya samu nasarar amfani da shi, babban kamfani a fagen POCT don amfanin ɗan adam, kuma yana da cikakkiyar fa'ida a cikin hako acid nucleic na wasu samfuran hadaddun (kamar Mycobacterium tarin fuka).
Don haka, ana ba da shawarar a zaɓi kayan gwaji ko kayan aikin da aka daidaita tare da matakin hako acid nucleic.kuma shi ne mafi kyau duka idan akwai wani ultrasonic hakar module.
3. Manual, Semi-atomatik da cikakken atomatik?
Wannan matsala ce ta farashin aiki da ingancin aiki.A halin yanzu, asibitocin dabbobi ba tare da isassun ma'aikata ba, da hakar acid nucleic da ganowa aiki ne da ke buƙatar wasu ƙwarewa da ƙwarewa.Babu shakka cewa cikakken atomatik hakar acid nucleic da injin ganowa shine cikakken zaɓi.
4. Ƙwaƙwalwar zafin jiki na dindindin ko ƙara girman zafin jiki?
Amsar haɓakawa shine hanyar gano acid nucleic, kuma fasahar ƙwararrun da ke cikin wannan hanyar haɗin gwiwar tana da sarkakiya.Kusan magana, ana amfani da enzymes don haɓaka acid nucleic.A cikin tsarin haɓakawa, ana gano siginar faɗaɗawa ko siginar walƙiya da aka haɗa.Gabaɗaya magana, da farko siginar fulawa ta bayyana, mafi girman abin da ake nufi da abun cikin samfurin.
Ƙwaƙwalwar zafin jiki na yau da kullun shine haɓakar acid nucleic a ƙayyadaddun zafin jiki, yayin da haɓakar zafin jiki mai canzawa shine haɓakawa ta zagaye-zagaye daidai gwargwado-ƙara-ƙasa-tsawo.An aiwatar da lokacin ƙara yawan zafin jiki akai-akai, yayin da lokacin ƙara yawan zafin jiki yana da matukar tasiri ta ƙimar zafin jiki da faɗuwar kayan aiki (a halin yanzu, masana'antun da yawa sun sami damar yin hawan keke 40 na haɓakawa cikin kusan mintuna 30).
Idan yanayin dakin gwaje-gwaje yana da kyau kuma tsarin yanki ya kasance mai tsauri, yana da kyau a ce daidaito tsakanin su biyun ba zai yi girma ba.Koyaya, haɓaka yanayin zafi mai canzawa zai haɗa ƙarin samfuran nucleic acid a cikin ɗan gajeren lokaci.Ga dakunan gwaje-gwaje ba tare da tsayayyen tsarin yanki ba da ƙwararrun ma'aikatan horarwa, haɗarin ɗigon aerosol na nucleic acid zai fi girma, tabbataccen ƙarya yana faruwa da zarar yayyo ya faru, kuma yana da matukar wahala a kawar da shi.
Bugu da ƙari, ƙara girman zafin jiki na yau da kullun yana da sauƙi ga haɓakawa mara ƙayyadaddun lokacin da samfurin ya kasance mai rikitarwa (madaidaicin zafin jiki na dangi yana da ƙasa, kuma mafi girman yanayin zafin tsawo, mafi kyawun ƙayyadaddun ƙayyadaddun ƙayyadaddun firam).
Dangane da fasahar zamani, haɓaka yanayin zafi mai canzawa ya fi dogaro.
5. Ta yaya za a guje wa haɗarin yabo na samfuran haɓaka acid nucleic?
A halin yanzu, masana'antun da yawa suna zaɓar nau'in nau'in PCR na gland a matsayin bututun amsawar nucleic acid, wanda aka rufe ta hanyar gogayya, da ƙarancin zafin jiki a cikin ƙarancin zafin jiki mai ma'ana a cikin madaidaicin zafin PCR ya kai digiri 90.
Centigrade .Tsarin maimaitawa na faɗaɗa tare da zafi da ƙanƙancewa tare da sanyi babban ƙalubale ne ga hatimin bututun PCR, kuma nau'in ƙwayar cuta na PCR yana da sauƙin haifar da zubewa.
Zai fi dacewa a ɗauki martanin tare da rufaffiyar kit/tube gaba ɗaya don gujewa ɗigowar samfurin amsawa.Zai zama cikakke idan za a iya samun aikin fitar da cikakkiyar kit ɗin da aka rufe don hakar acid nucleic da ganowa.
Don haka Sabuwar Tech sabon cikkaken hako acid nucleic atomatik da injin ganowa yana da mafi kyawun zaɓi guda biyar na sama.
Lokacin aikawa: Agusta-09-2023
